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验证数据展示

  • WB analysis using 31745-1-AP

    WB analysis using 31745-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31745-1-AP (CD11b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 31745-1-AP

    IF Staining of RAW 264.7 using 31745-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD11b antibody (31745-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of BV-2 using 31745-1-AP

    IF Staining of BV-2 using 31745-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BV-2 cells using CD11b antibody (31745-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD11b Polyclonal antibody

CD11b Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31745-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

CR3, CR3A, integrin alpha M, Itgam, Ly 40

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJ774A.1 cells, RAW 264.7 cells
Positive IF/ICC detected inRAW 264.7 cells, BV-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31745-1-AP targets CD11b in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Ag36145

Product name: Recombinant mouse Itgam protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 925-1105 aa of BC156991

Sequence: AIYMIVTSDESSIRYLNFTASEMTSKVIQHQYQFNNLGQRSLPVSVVFWIPVQINNVTVWDHPQVIFSQNLSSACHTEQKSPPHSNFRDQLERTPVLNCSVAVCKRIQCDLPSFNTQEIFNVTLKGNLSFDWYIKTSHGHLLLVSSTEILFNDSAFALLPGQESYVRSKTETKVEPYEVHN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrin alpha M
别名 CR3, CR3A, integrin alpha M, Itgam, Ly 40
计算分子量127 kDa
观测分子量150 kDa
GenBank蛋白编号BC156991
基因名称 CD11b
Gene ID (NCBI) 16409
RRIDAB_3670104
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDE9Q604
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

实验方案

Product Specific Protocols
IF protocol for CD11b antibody 31745-1-APDownload protocol
WB protocol for CD11b antibody 31745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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