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验证数据展示

  • IHC staining of human colon using 84259-3-RR

    IHC staining of human colon using 84259-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84259-3-RR (CD117/c-Kit antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD117/c-kit antibody included
  • IHC staining of human colon using 84259-3-RR

    IHC staining of human colon using 84259-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84259-3-RR (CD117/c-Kit antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD117/c-kit antibody included
  • IF Staining of Mo7e using 84259-3-RR

    IF Staining of Mo7e using 84259-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Mo7e cells using CD117/c-Kit antibody (84259-3-RR, Clone: 241312A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84259-3-RR

    Affinity and Kinetic Characterization of 84259-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84259-3-RR against Human CD117/c-Kit were performed. The affinity constant is 0.519 nM.

CD117/c-Kit Recombinant antibody

CD117/c-Kit Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84259-3-RR

产品说明书

CloneNo. 241312A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

CD117, KIT, EC:2.7.10.1, c-Kit, C Kit

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMo7e cells
Planning an IHC experiment? We recommend our IHCeasy CD117/c-kit Ready-To-Use IHC Kit. CD117/c-kit primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84259-3-RR targets CD117/c-Kit in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
别名 CD117, KIT, EC:2.7.10.1, c-Kit, C Kit
计算分子量 976 aa, 110 kDa
GenBank蛋白编号BC071593
基因名称 CD117
Gene ID (NCBI) 3815
RRIDAB_3671806
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10721
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

实验方案

Product Specific Protocols
IHC protocol for CD117/c-Kit antibody 84259-3-RRDownload protocol
IF protocol for CD117/c-Kit antibody 84259-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols