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验证数据展示

  • WB analysis using RMX00094

    WB analysis using RMX00094

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00094 (CD107b / LAMP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using RMX00094

    IF Staining of A549 using RMX00094

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CD107b / LAMP2 antibody (RMX00094) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IHC staining of human liver using RMX00094

    IHC staining of human liver using RMX00094

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using RMX00094 (CD107b / LAMP2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LAMP2 antibody included
  • WB analysis of HeLa using RMX00094

    WB analysis of HeLa using RMX00094

    WB result of CD107b / LAMP2 antibody (RMX00094; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD107b / LAMP2 transfected HeLa cells.

Multi-rAb® CD107b / LAMP2 Multi-Recombinant antibody

CD107b / LAMP2 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00094

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD107b, LAMP2, CD107 antigen-like family member B, LAMP 2, LAMP-2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00094 targets CD107b / LAMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

FusionProtein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 CD107b, LAMP2, CD107 antigen-like family member B, LAMP 2, LAMP-2
计算分子量45 kDa
观测分子量100 kDa~ 120 kDa
GenBank蛋白编号NM_002294.3
基因名称 LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP13473-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.

实验方案

Product Specific Protocols
IF protocol for CD107b / LAMP2 antibody RMX00094Download protocol
IHC protocol for CD107b / LAMP2 antibody RMX00094Download protocol
WB protocol for CD107b / LAMP2 antibody RMX00094Download protocol
Standard Protocols
Click here to view our Standard Protocols
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