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验证数据展示

  • WB analysis using 32148-1-AP

    WB analysis using 32148-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32148-1-AP (CD107b / LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 32148-1-AP

    IF Staining of NIH/3T3 using 32148-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CD107b / LAMP2 antibody (32148-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

CD107b / LAMP2 Polyclonal antibody

CD107b / LAMP2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32148-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, ELISA

别名

Lamp2, CD107b, Mac3, Lamp 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32148-1-AP targets CD107b / LAMP2 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 Lamp2, CD107b, Mac3, Lamp 2
计算分子量46 kDa
观测分子量70-100 kDa
GenBank蛋白编号NM_001017959.2
基因名称 CD107b / LAMP2
Gene ID (NCBI) 16784
RRIDAB_3670208
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP17047-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP2 is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
WB protocol for CD107b / LAMP2 antibody 32148-1-APDownload protocol
IF protocol for CD107b / LAMP2 antibody 32148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols