Anti-Human CD107b / LAMP2 (H4B4)

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. 65053-1-Ig

产品说明书

CloneNo. H4B4

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

IF/ICC, FC (Intra) and More (1)

CD107b, LAMP2, LAMP-2, LAMP 2, H4B4

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive IF/ICC detected inMCF-7 cells, U-251 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

65053-1-Ig targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性human
免疫原 Human adherent peripheral blood cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 CD107b, LAMP2, LAMP-2, LAMP 2, H4B4
计算分子量 45 kDa
GenBank蛋白编号BC002965
基因名称 LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
RRIDAB_2881469
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13473
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
IF protocol for CD107b / LAMP2 antibody 65053-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Biol Chem

Calmodulin enhances mTORC1 signaling by preventing TSC2-Rheb binding

Authors - Yuna Amemiya
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