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验证数据展示

  • WB analysis using RMX00025

    WB analysis using RMX00025

    Untreated and PNGaseF-treated HeLa cell lysates were subjected to SDS PAGE followed by western blot with RMX00025 (CD107a / LAMP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using RMX00025

    IP experiment of HeLa using RMX00025

    IP result of anti-CD107a / LAMP1 (IP:RMX00025, 4ug; Detection:RMX00025 1:20000) with HeLa cells lysate 1200 ug.

  • IF Staining of HepG2 using RMX00025

    IF Staining of HepG2 using RMX00025

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CD107a / LAMP1 antibody (RMX00025) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Multi-rAb® CD107a / LAMP1 Multi-Recombinant antibody

CD107a / LAMP1 Multi-Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. RMX00025

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, LAMP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00025 targets CD107a / LAMP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 lysosomal-associated membrane protein 1
别名 CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, LAMP 1
计算分子量45 kDa
观测分子量 40-48 kDa, 90-120 kDa
GenBank蛋白编号NM_005561.4
基因名称 LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP11279-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

实验方案

Product Specific Protocols
WB protocol for CD107a / LAMP1 antibody RMX00025Download protocol
IF protocol for CD107a / LAMP1 antibody RMX00025Download protocol
IP protocol for CD107a / LAMP1 antibody RMX00025Download protocol
Standard Protocols
Click here to view our Standard Protocols