验证数据展示
经过测试的应用
| Positive FC detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65049-1-Ig targets CD106 in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | Human |
| 免疫原 | N/A 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vascular cell adhesion molecule 1 |
| 别名 | CD106, DKFZp779G2333, INCAM 100, V CAM 1, VCAM 1, VCAM1 |
| 计算分子量 | 739 aa, 81 kDa |
| GenBank蛋白编号 | BC017276 |
| 基因名称 | VCAM-1 |
| Gene ID (NCBI) | 7412 |
| RRID | AB_2918361 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P19320 |
| 储存缓冲液 | Borate buffered saline , pH 8.2 |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CD106 antibody 65049-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |