验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse liver tissue |
| Positive FC detected in | bEnd.3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
65048-1-Ig targets Endoglin/CD105 in IF-P, FC applications and shows reactivity with mouse samples.
| 经测试应用 | IF-P, FC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | mouse |
| 文献引用反应性 | mouse |
| 免疫原 | Inflamed mouse skin 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | endoglin |
| 别名 | CD105, Edg, endoglin, Eng, MJ7/18 |
| GenBank蛋白编号 | BC029080 |
| 基因名称 | CD105 |
| Gene ID (NCBI) | 13805 |
| RRID | AB_2918360 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | Q63961 |
| 储存缓冲液 | Borate buffered saline, pH 8.2. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Endoglin/CD105 antibody 65048-1-Ig | Download protocol |
| FC protocol for Endoglin/CD105 antibody 65048-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |