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验证数据展示

  • WB analysis using 27516-1-AP (same clone as 27516-1-PBS)

    WB analysis using 27516-1-AP (same clone as 27516-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27516-1-AP (CD100 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 27516-1-AP (same clone as 27516-1-PBS)

    WB analysis of HeLa using 27516-1-AP (same clone as 27516-1-PBS)

    WB result of CD100 antibody (27516-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD100 transfected HeLa cells. This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 27516-1-AP (same clone as 27516-1-PBS)

    IHC staining of human tonsillitis using 27516-1-AP (same clone as 27516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 27516-1-AP (same clone as 27516-1-PBS)

    IHC staining of human tonsillitis using 27516-1-AP (same clone as 27516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CD100 Polyclonal antibody, PBS Only

CD100 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27516-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

SEMA4D, A8, CD 100, CD100 antigen, SEM4D

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

27516-1-PBS targets CD100 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26461

Product name: Recombinant human CD100 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 756-862 aa of BC054500

Sequence: YKGYLPRQCLKFRSALLIGKKKPKSDFCDREQSLKETLVEPGSFSQQNGEHPKPALDTGYETEQDTITSKVPTDREDSQRIDDLSARDKPFDVKCELKFADSDADGD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
别名 SEMA4D, A8, CD 100, CD100 antigen, SEM4D
计算分子量 96 kDa
观测分子量 96 kDa
GenBank蛋白编号BC054500
基因名称 SEMA4D/CD100
Gene ID (NCBI) 10507
RRIDAB_2880896
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92854
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

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