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验证数据展示

  • WB analysis using 27516-1-AP

    WB analysis using 27516-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27516-1-AP (CD100 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27516-1-AP

    IHC staining of human tonsillitis using 27516-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27516-1-AP

    IHC staining of human tonsillitis using 27516-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD100 Polyclonal antibody

CD100 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27516-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IHC detected inhuman tonsillitis tissue, human colon tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27516-1-AP targets CD100 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 CD100 fusion protein Ag26461 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
别名 A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D
计算分子量 96 kDa
观测分子量 96 kDa
GenBank蛋白编号BC054500
基因名称 SEMA4D/CD100
Gene ID (NCBI) 10507
RRIDAB_2880896
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92854
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

实验方案

Product Specific Protocols
WB protocol for CD100 antibody 27516-1-APDownload protocol
IHC protocol for CD100 antibody 27516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols