- Featured Product
- KD/KO Validated
CCT8 Polyclonal antibody
CCT8 Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, mouse kidney tissue, HEK-293 cells, COLO 320 cells, HeLa cells, Jurkat cells, Raji cells |
Positive IP detected in | Jurkat cells |
Positive IF detected in | SW480 cells, MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12263-1-AP targets CCT8 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IP, WB, ELISA |
Cited Applications | WB, IP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | CCT8 fusion protein Ag2926 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | chaperonin containing TCP1, subunit 8 (theta) |
Synonyms | C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta |
Calculated Molecular Weight | 548 aa, 60 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC012584 |
Gene Symbol | CCT8 |
Gene ID (NCBI) | 10694 |
RRID | AB_906331 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P50990 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CCT8 antibody 12263-1-AP | Download protocol |
IF protocol for CCT8 antibody 12263-1-AP | Download protocol |
IP protocol for CCT8 antibody 12263-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan. | ||
Proc Natl Acad Sci U S A BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. | ||