验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HEK-293 cells, HeLa cells, mouse heart tissue |
| Positive IHC detected in | human liver cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21524-1-AP targets CCT4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16128 Product name: Recombinant human CCT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 108-407 aa of BC106934 Sequence: SVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chaperonin containing TCP1, subunit 4 (delta) |
| 别名 | CCTD, CCT-delta, Chaperonin containing T-complex polypeptide 1 subunit 4, SRB, Stimulator of TAR RNA-binding |
| 计算分子量 | 539 aa, 58 kDa |
| 观测分子量 | 58-60 kDa |
| GenBank蛋白编号 | BC106934 |
| 基因名称 | CCT4 |
| Gene ID (NCBI) | 10575 |
| RRID | AB_10733520 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P50991 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCT4 (Chaperonin containing TCP1 complex 4), also known as TCP-1-delta, is a recent identified member of the family, that serves as a component of the chaperonin-containing TCP1 complex (TRiC) and also functions as a monomer. CCT4 has been shown to influence the cytoskeletal structure and regulate F-actin organization independently of TRiC (PMID: 41104595). CCT4 was highly expressed in human ESCC tissues and cell lines, and closely related to the poor prognosis (PMID: 36102200).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCT4 antibody 21524-1-AP | Download protocol |
| WB protocol for CCT4 antibody 21524-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.
| ||
Mol Ther Nucleic Acids hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4. | ||
Pathog Dis Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT. | ||
Medicine (Baltimore) Chaperonin-containing TCP-1 subunit genes are potential prognostic biomarkers and are correlated with Th2 cell infiltration in lung adenocarcinoma: An observational study | ||
