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验证数据展示

  • WB analysis using 28644-1-AP

    WB analysis using 28644-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28644-1-AP (C1orf96 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28644-1-AP

    IHC staining of mouse brain using 28644-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28644-1-AP (CCSAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCSAP antibody included
  • IHC staining of rat brain using 28644-1-AP

    IHC staining of rat brain using 28644-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28644-1-AP (CCSAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCSAP antibody included

CCSAP Polyclonal antibody

CCSAP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28644-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

C1orf96, Centriole, cilia and spindle-associated protein, CSAP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CCSAP Ready-To-Use IHC Kit. CCSAP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28644-1-AP targets CCSAP in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Ag23785

Product name: Recombinant human C1orf96 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-270 aa of BC015419

Sequence: MSPGSGVKSEYMKRYQEPRWEEYGPCYRELLHYRLGRRLLEQAHAPWLWDDWGPAGSSEDSASSESSGAGGPAPRCAPPSPPPPVEPATQEEAERRARGAPEEQDAEAGDAEAEDAEDAALPALPVKDVEDKPEQQTRTRETDKSPTSTEPRQQPSALFARGNRKAVKSPQRSSSKIKENKHPFALYGWGEKQTDTGSQKTHNVCASAPVHEIHESALRAKNRRQVEKRKLVAQRQRAHSVDVEKNRKMKASSSENPWMTEYMRCYSARA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 96
别名 C1orf96, Centriole, cilia and spindle-associated protein, CSAP
观测分子量30-36 kDa
GenBank蛋白编号BC015419
基因名称 CCSAP/C1orf96
Gene ID (NCBI) 126731
RRIDAB_3086074
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6IQ19
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCSAP (Centriole, cilia and spindle-associated protein) colocalizes with polyglutamylated tubulin to centrioles, spindle microtubules, and cilia in human tissue culture cells (PMID: 22493317). Plays a role in microtubule (MT) stabilization and this stabilization involves the maintenance of NUMA1 at the spindle poles. It can be detected as around 30-36 kDa.

实验方案

Product Specific Protocols
IHC protocol for CCSAP antibody 28644-1-APDownload protocol
WB protocol for CCSAP antibody 28644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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