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验证数据展示

  • WB analysis using 68341-1-Ig (same clone as 68341-1-PBS)

    WB analysis using 68341-1-Ig (same clone as 68341-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68341-1-Ig (CCS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    WB analysis of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    WB result of CCS antibody (68341-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCS transfected HepG2 cells. This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    IF Staining of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCS antibody (68341-1-Ig, Clone: 3F5E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68341-1-Ig (same clone as 68341-1-PBS)

    IHC staining of mouse brain using 68341-1-Ig (same clone as 68341-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68341-1-Ig (CCS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CCS Monoclonal antibody, PBS Only

CCS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68341-1-PBS

产品说明书

CloneNo. 3F5E5

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CCS

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68341-1-PBS targets CCS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 CCS fusion protein Ag18860 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 copper chaperone for superoxide dismutase
别名 CCS
计算分子量 274 aa, 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC105016
基因名称 CCS
Gene ID (NCBI) 9973
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14618
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.