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验证数据展示

  • WB analysis using 68341-1-Ig (same clone as 68341-1-PBS)

    WB analysis using 68341-1-Ig (same clone as 68341-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68341-1-Ig (CCS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    WB analysis of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    WB result of CCS antibody (68341-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCS transfected HepG2 cells. This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    IF Staining of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCS antibody (68341-1-Ig, Clone: 3F5E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68341-1-Ig (same clone as 68341-1-PBS)

    IHC staining of mouse brain using 68341-1-Ig (same clone as 68341-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68341-1-Ig (CCS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CCS Monoclonal antibody, PBS Only

CCS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68341-1-PBS

产品说明书

CloneNo. 3F5E5

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CCS

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68341-1-PBS targets CCS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag18860

Product name: Recombinant human CCS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-274 aa of BC105016

Sequence: MASDSGNQGTLCTLEFAVQMTCQSCVDAVRKSLQGVAGVQDVEVHLEDQMVLVHTTLPSQEVQALLEGTGRQAVLKGMGSGQLQNLGAAVAILGGPGTVQGVVRFLQLTPERCLIEGTIDGLEPGLHGLHVHQYGDLTNNCNSCGNHFNPDGASHGGPQDSDRHRGDLGNVRADADGRAIFRMEDEQLKVWDVIGRSLIIDEGEDDLGRGGHPLSKITGNSGERLACGIIARSAGLFQNPKQICSCDGLTIWEERGRPIAGKGRKESAQPPAHL

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 copper chaperone for superoxide dismutase
别名 CCS
计算分子量 274 aa, 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC105016
基因名称 CCS
Gene ID (NCBI) 9973
RRIDAB_3085065
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14618
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.

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