CCS Polyclonal antibody

CCS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 22802-1-AP

Print datasheet

Synonyms

CCS, SOD 4, SOD4, Superoxide Dismutase 4



经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

发表文章中的应用

WBSee 5 publications below

产品信息

22802-1-AP targets CCS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen CCS fusion protein Ag18850 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name copper chaperone for superoxide dismutase
Synonyms CCS, SOD 4, SOD4, Superoxide Dismutase 4
Calculated molecular weight 274 aa, 29 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC105016
Gene symbol CCS
UniprotO14618
Gene ID (NCBI) 9973
RRIDAB_2879172
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.

实验方案

Product Specific Protocols
WB protocol for CCS antibody 22802-1-APDownload protocol
IHC protocol for CCS antibody 22802-1-APDownload protocol
IF protocol for CCS antibody 22802-1-APDownload protocol
IP protocol for CCS antibody 22802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Stem Cell

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Authors - Rashim Pal Singh
humanWB

Neurobiol Dis

The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease.

Authors - Nadine Bakkar
mouseWB

Sci Rep

S-acylation of SOD1, CCS, and a stable SOD1-CCS heterodimer in human spinal cords from ALS and non-ALS subjects.

Authors - Sarah E Antinone
humanWB

Br J Cancer

AMPK phosphorylates and stabilises copper transporter 1 to synergise metformin and copper chelator for breast cancer therapy

Authors - Xiaomei Zhang
mouseWB

Mol Med Rep

Artemether ameliorates adriamycin induced cardiac atrophy in mice

Authors - Wenci Weng