CCS Polyclonal antibody
CCS Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse brain tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
22802-1-AP targets CCS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF, IHC, IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | CCS fusion protein Ag18850 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | copper chaperone for superoxide dismutase |
| Synonyms | CCS, SOD 4, SOD4, Superoxide Dismutase 4 |
| Calculated molecular weight | 274 aa, 29 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC105016 |
| Gene symbol | CCS |
| Uniprot | O14618 |
| Gene ID (NCBI) | 9973 |
| RRID | AB_2879172 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCS antibody 22802-1-AP | Download protocol |
| IHC protocol for CCS antibody 22802-1-AP | Download protocol |
| IF protocol for CCS antibody 22802-1-AP | Download protocol |
| IP protocol for CCS antibody 22802-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal. | ||
Neurobiol Dis The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease. | ||
Sci Rep S-acylation of SOD1, CCS, and a stable SOD1-CCS heterodimer in human spinal cords from ALS and non-ALS subjects. | ||
Br J Cancer AMPK phosphorylates and stabilises copper transporter 1 to synergise metformin and copper chelator for breast cancer therapy | ||
