验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87796-1-RR targets CCRL1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) receptor-like 1 |
| 别名 | ACKR4, Atypical chemokine receptor 4, C-C chemokine receptor type 11, CC chemokine receptor-like 1, C-C CKR-11 |
| 计算分子量 | 350 aa, 40 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC069438 |
| 基因名称 | CCRL1 |
| Gene ID (NCBI) | 51554 |
| RRID | AB_3745754 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NPB9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCRL1/ACKR4 is an atypical chemokine receptor that functions primarily as a chemokine scavenger, binding CCL19, CCL21, CCL25, and CXCL13 to shape local chemokine gradients rather than inducing classical GPCR-mediated chemotaxis. Expressed in lymphatic endothelium, epithelial barriers, and stromal compartments, ACKR4 plays a key role in dendritic cell trafficking, lymphocyte positioning, and tissue immune homeostasis. Dysregulation of ACKR4 can alter barrier immunity, inflammatory responses, and tumor immune organization, highlighting its importance as a non-signaling regulator of chemokine networks.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCRL1 antibody 87796-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
