验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21018-1-AP targets CCR9 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag15268 Product name: Recombinant human CCR9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 265-369 aa of BC069678 Sequence: LSQFPYNCILLVQTIDAYAMFISNCAVSTNIDICFQVTQTIAFFHSCLNPVLYVFVGERFRRDLVKTLKNLGCISQAQWVSFTRREGSLKLSSMLLETTSGALSL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) receptor 9 |
| 别名 | C C chemokine receptor type 9, C C CKR 9, CC CKR 9, CCR 9, CDw199 |
| 计算分子量 | 369 aa, 42 kDa |
| 观测分子量 | 38-40 kDa |
| GenBank蛋白编号 | BC069678 |
| 基因名称 | CCR9 |
| Gene ID (NCBI) | 10803 |
| RRID | AB_3742013 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P51686 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCR9 is a Gi-coupled chemokine receptor that directs T cell migration to the small intestine through interaction with CCL25, playing an essential role in thymocyte development and mucosal immune surveillance. Dysregulation of CCR9 signaling contributes to intestinal inflammatory diseases and hematologic malignancies, highlighting its importance in tissue-specific lymphocyte homing and immune regulation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCR9 antibody 21018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
