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CCR7 Polyclonal antibody

CCR7 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

FC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 55425-1-AP

Print datasheet

Synonyms

BLR2, C C chemokine receptor type 7, C C CKR 7, CC CKR 7, CCR 7, CCR7, CD197, CDw197, CMKBR7, EBI1, EVI1, MIP 3 beta receptor

验证数据展示

  • FC experiment of K-562 using 55425-1-AP

    FC experiment of K-562 using 55425-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CCR7 (55425-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human tonsillitis using 55425-1-AP

    IHC staining of human tonsillitis using 55425-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55425-1-AP (CCR7 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 55425-1-AP

    IHC staining of human tonsillitis using 55425-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55425-1-AP (CCR7 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

55425-1-AP targets CCR7 in FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, ELISA
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-C motif) receptor 7
Synonyms BLR2, C C chemokine receptor type 7, C C CKR 7, CC CKR 7, CCR 7, CCR7, CD197, CDw197, CMKBR7, EBI1, EVI1, MIP 3 beta receptor
Calculated Molecular Weight 43 kDa
GenBank Accession NumberNM_001838
Gene Symbol CCR7
Gene ID (NCBI) 1236
RRIDAB_2881325
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32248
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-C chemokine receptor type 7 (CCR7) , which belongs to the G-protein coupled receptor 1 family, is also a receptor for the MIP-3-beta chemokine, and probablely acts as mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. CCR7 is specially expressed in various lymphoid tissues and activated B- and T-lymphocytes, and can be strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

实验方案

Product Specific Protocols
IHC protocol for CCR7 antibody 55425-1-APDownload protocol
FC protocol for CCR7 antibody 55425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Adv Sci (Weinh)

Material Stiffness in Cooperation with Macrophage Paracrine Signals Determines the Tenogenic Differentiation of Mesenchymal Stem Cells

Authors - Renwang Sheng
View Article
humanIHC

Nat Commun

A single-cell atlas of the multicellular ecosystem of primary and metastatic hepatocellular carcinoma.

Authors - Yiming Lu
View Article