验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 3 publications below |
| IF | See 4 publications below |
产品信息
17476-1-AP targets CCR5 in IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, macaque |
| 免疫原 |
CatNo: Ag11493 Product name: Recombinant human CCR5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 257-352 aa of BC038398 Sequence: LNTFQEFFGLNNCSSSNRLDQAMQVTETLGMTHCCINPIIYAFVGEKFRNYLLVFFQKHIAKRFCKCCSIFQQEAPERASSVYTRSTGEQEISVGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) receptor 5 |
| 别名 | C C chemokine receptor type 5, C C CKR 5, CC CKR 5, CCCKR5, CCR 5, CCR5, CD195, CHEMR13, CKR 5, CKR5, CMKBR5, HIV 1 fusion coreceptor, IDDM22 |
| 计算分子量 | 352 aa, 41 kDa |
| GenBank蛋白编号 | BC038398 |
| 基因名称 | CCR5 |
| Gene ID (NCBI) | 1234 |
| RRID | AB_10895966 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51681 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCR5 (also known as CD195) is a member of the beta chemokine receptor family. This protein is expressed by T cells and macrophages and is known to be an important co-receptor for macrophage-tropic viruses, including HIV, to enter host cells. The natural chemokine ligands that bind to CCR5 include MIP-1-alpha, MIP-1-beta, MCP-2, and RANTES.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCR5 antibody 17476-1-AP | Download protocol |
| FC protocol for CCR5 antibody 17476-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Nat Immunol Targeting regulator of G protein signaling 1 in tumor-specific T cells enhances their trafficking to breast cancer. | ||
J Biol Chem Regulation of C-C chemokine receptor 5 (CCR5) stability by Lys197 and by transmembrane protein aptamers that target it for lysosomal degradation. | ||
Sci Rep PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats. | ||
Retrovirology CCR5 editing by Staphylococcus aureus Cas9 in human primary CD4+ T cells and hematopoietic stem/progenitor cells promotes HIV-1 resistance and CD4+ T cell enrichment in humanized mice. | ||
Acta Trop Mechanism of Lian Hua Qing Wen capsules regulates the inflammatory response caused by M1 macrophage based on cellular experiments and computer simulations |