验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse skeletal muscle tissue, HEK-293 cells, rat skeletal muscle tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
RMX00052 targets CCR2a-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Multi-Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) receptor 2 |
| 别名 | CCR2, C C chemokine receptor type 2, C C CKR 2, C-C chemokine receptor type 2, CC CKR 2 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 37-42 kDa |
| GenBank蛋白编号 | NM_000647 |
| 基因名称 | CCR2 |
| Gene ID (NCBI) | 729230 |
| RRID | AB_3750104 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | N/A |
| UNIPROT ID | P41597 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CCR2a-specific antibody RMX00052 | Download protocol |
| IHC protocol for CCR2a-specific antibody RMX00052 | Download protocol |
| WB protocol for CCR2a-specific antibody RMX00052 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
