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验证数据展示

  • WB analysis using 21860-1-AP

    WB analysis using 21860-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21860-1-AP (cyclin T2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 21860-1-AP

    IHC staining of mouse brain using 21860-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 4010x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 21860-1-AP

    IHC staining of rat brain using 21860-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 21860-1-AP

    IHC staining of rat brain using 21860-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

cyclin T2 Polyclonal antibody

cyclin T2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21860-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CCNT2, CycT2, Cyclin-T2, Cyclin T

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21860-1-AP targets cyclin T2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 cyclin T2 fusion protein Ag16514 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin T2
别名 CCNT2, CycT2, Cyclin-T2, Cyclin T
计算分子量 730 aa, 81 kDa
观测分子量 70 kDa
GenBank蛋白编号BC114366
基因名称 Cyclin T2
Gene ID (NCBI) 905
RRIDAB_3085675
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60583
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin T2 (CCNT2) belongs to the cyclin family. CCNT2 is regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II)  (PMID: 9499409, PMID: 15563843). Cyclin T2 is expressed at a relatively high level in freshly isolated monocytes and its level remains constant when the cells are induced to undergo macrophage differentiation (PMID: 21791050). In contrast, Cyclin T1 is expressed at low levels in monocytes and it is strongly up-regulated by a post-transcriptional mechanism when the cells are induced to differentiate to macrophages.

实验方案

Product Specific Protocols
WB protocol for cyclin T2 antibody 21860-1-APDownload protocol
IHC protocol for cyclin T2 antibody 21860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols