验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, HeLa cells, U-251 cells, human placenta tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11212-1-AP targets CCNL2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CCNL2 fusion protein Ag1710 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cyclin L2 |
别名 | ANIA 6B, CCNL2, CCNM, cyclin L2, HCLA ISO, HLA ISO, PCEE, SB138 |
计算分子量 | 58 kDa |
观测分子量 | 28 kDa |
GenBank蛋白编号 | BC016333 |
基因名称 | CCNL2 |
Gene ID (NCBI) | 81669 |
RRID | AB_2073623 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96S94 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCNL2 (Cyclin-L2) is also named as SB138 and Paneth cell-enhanced expression protein. Human cyclin L2 is a novel member of the cyclin family. Human cyclin L2 shares significant homology to cyclin L1, K, T1, T2, and C, which are involved in transcriptional regulation via phosphorylation of the C-terminal domain of RNA polymerase II (PMID: 14684736). Cyclin L2 co-localizes with splicing factors SC-35 and 9G8 within nuclear speckles and that it associates with hyperphosphorylated, but not hypophosphorylated, RNA polymerase II and CDK p110 PITSLRE kinase via its N-terminal cyclin domains (PMID: 14684736). Human cyclin L2 is expressed ubiquitously in normal human tissues and tumor cells.
实验方案
Product Specific Protocols | |
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WB protocol for CCNL2 antibody 11212-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |