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验证数据展示

  • WB analysis using 15694-1-AP

    WB analysis using 15694-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15694-1-AP (CCNJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 15694-1-AP

    IHC staining of rat kidney using 15694-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15694-1-AP (CCNJ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCNJ Polyclonal antibody

CCNJ Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15694-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Cyclin-J, cyclin J

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15694-1-AP targets CCNJ in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CCNJ fusion protein Ag8273 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin J
别名 Cyclin-J, cyclin J
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC043175
基因名称 CCNJ
Gene ID (NCBI) 54619
RRIDAB_3085470
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T5M9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCNJ (Cyclin-J) Belongs to the Cyclin family. Cyclin J is expressed in early Drosophila embryos, and it is essential for mediating intestinal epithelial cell recovery from bacterial pore-forming toxin attack in both Drosophila and mice. Upon TLR and IFN stimulation, cyclin J restrained macrophage inflammatory responses irrespective of the cell cycle control. Cyclin J interacted with CDKs and facilitated the phosphorylation of a set of CDK substrates, including FoxK1 and Drp1. Phosphorylation inhibited FoxK1-mediated expression of glycolytic genes by reducing FoxK1 nuclear localization. Cyclin J-CDK-dependent Drp1 phosphorylation also resulted in mitochondrial fission and the suppression of ROS production (PMID: 35412849).

实验方案

Product Specific Protocols
WB protocol for CCNJ antibody 15694-1-APDownload protocol
IHC protocol for CCNJ antibody 15694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols