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验证数据展示

  • WB analysis using 10718-1-AP

    WB analysis using 10718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10718-1-AP (Cyclin H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10718-1-AP

    IHC staining of human breast cancer using 10718-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10718-1-AP (Cyclin H antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10718-1-AP

    IHC staining of human breast cancer using 10718-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10718-1-AP (Cyclin H antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 10718-1-AP

    IP experiment of HeLa using 10718-1-AP

    IP result of anti-Cyclin H (IP:10718-1-AP, 4ug; Detection:10718-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • IF Staining of A431 using 10718-1-AP

    IF Staining of A431 using 10718-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cyclin H antibody (10718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 10718-1-AP

    FC experiment of A431 using 10718-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Cyclin H Polyclonal antibody (10718-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Cyclin H Polyclonal antibody

Cyclin H Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10718-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

CCNH, p37, p34, MO15-associated protein, MO15 associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, A375 cells, mouse brain tissue, HuH-7 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10718-1-AP targets Cyclin H in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cyclin H fusion protein Ag1088 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin H
别名 CCNH, p37, p34, MO15-associated protein, MO15 associated protein
计算分子量 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC005280
基因名称 Cyclin H
Gene ID (NCBI) 902
RRIDAB_2073749
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51946
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Cyclin H antibody 10718-1-APDownload protocol
IHC protocol for Cyclin H antibody 10718-1-APDownload protocol
IF protocol for Cyclin H antibody 10718-1-APDownload protocol
IP protocol for Cyclin H antibody 10718-1-APDownload protocol
FC protocol for Cyclin H antibody 10718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols