验证数据展示

WB analysis using 11554-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 11554-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11554-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11554-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HeLa using 11554-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclin E1 (11554-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
FC experiment of MCF-7 using 11554-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin E1 (11554-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
WB analysis of HeLa using 11554-1-AP
Non-treated HeLa and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.
IP experiment of HeLa using 11554-1-AP
IP result of anti-Cyclin E1 (IP:11554-1-AP, 4ug; Detection:11554-1-AP 1:2000) with HeLa cells lysate 1600 ug.

Featured Product
KD/KO Validated

Cyclin E1 Polyclonal antibody

Cyclin E1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 11554-1-AP

产品说明书

引用文献(425)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (4)

应用

WB, IHC, FC (Intra), IP, ELISA and More (2)

别名

CCNE1, CCNE, Cyclin E, G1/S specific cyclin E1, G1/S-specific cyclin-E1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (425)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HT-29 cells, NIH/3T3 cells, mouse heart tissue, HeLa cells, Jurkat cells, human lung tissue, MCF-7 cells, HepG2 cells, K-562 cells, human placenta tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse testis tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells, MCF-7 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 391 publications below
IHC	See 17 publications below
IF	See 1 publications below
CoIP	See 1 publications below

产品信息

11554-1-AP targets Cyclin E1 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, CoIP
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat, pig, zebrafish, artemia sinica
免疫原	CatNo: Ag2110 Product name: Recombinant human Cyclin E protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-410 aa of BC035498 Sequence: ANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	cyclin E1
别名	CCNE1, CCNE, Cyclin E, G1/S specific cyclin E1, G1/S-specific cyclin-E1
计算分子量	410 aa, 47 kDa
观测分子量	47 kDa
GenBank蛋白编号	BC035498
基因名称	Cyclin E1
Gene ID (NCBI)	898
RRID	AB_2071066
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P24864
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).

实验方案

Product Specific Protocols
FC protocol for Cyclin E1 antibody 11554-1-AP	Download protocol
IHC protocol for Cyclin E1 antibody 11554-1-AP	Download protocol
IP protocol for Cyclin E1 antibody 11554-1-AP	Download protocol
WB protocol for Cyclin E1 antibody 11554-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Science In silico protein interaction screening uncovers DONSON's role in replication initiation Authors - Yang Lim View Article
human	WB	Mol Cancer Circular RNA BCRC-3 suppresses bladder cancer proliferation through miR-182-5p/p27 axis. Authors - Fei Xie View Article
human	WB	Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer. Authors - Xi Jin View Article
human	WB	Mol Cancer ZNF460-mediated circRPPH1 promotes TNBC progression through ITGA5-induced FAK/PI3K/AKT activation in a ceRNA manner Authors - Chuanpeng Zhang View Article
human	WB	Cancer Commun (Lond) IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma. Authors - Liang Wang View Article
mouse	WB	Nat Immunol tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis Authors - Yongbo Liu View Article

验证数据展示

WB analysis using 11554-1-AP

IHC staining of mouse testis using 11554-1-AP

IHC staining of human placenta using 11554-1-AP

IHC staining of human placenta using 11554-1-AP

FC experiment of HeLa using 11554-1-AP

FC experiment of MCF-7 using 11554-1-AP

WB analysis of HeLa using 11554-1-AP

IP experiment of HeLa using 11554-1-AP

Cyclin E1 Polyclonal antibody

Cyclin E1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

In silico protein interaction screening uncovers DONSON's role in replication initiation

Circular RNA BCRC-3 suppresses bladder cancer proliferation through miR-182-5p/p27 axis.

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

ZNF460-mediated circRPPH1 promotes TNBC progression through ITGA5-induced FAK/PI3K/AKT activation in a ceRNA manner

IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma.

tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis

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