验证数据展示
经过测试的应用
| Positive WB detected in | rat kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33150-1-AP targets CCNB1IP1 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag38148 Product name: Recombinant human CCNB1IP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-277 aa of BC004435 Sequence: MSLCEDMLLCNYRKCRIKLSGYAWVTACSHIFCDQHGSGEFSRSPAICPACNSTLSGKLDIVRTELSPSEEYKAMVLAGLRPEIVLDISSRALAFWTYQVHQERLYQEYNFSKAEGHLKQMEKIYTQQIQSKDVELTSMKGEVTSMKKVLEEYKKKFSDISEKLMERNRQYQKLQGLYDSLRLRNITIANHEGTLEPSMIAQSGVLGFPLGNNSKFPLDNTPVRNRGDGDGDFQFRPFFAGSPTAPEPSNSFFSFVSPSRELEQQQVSSRAFKVKRI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin B1 interacting protein 1 |
| 别名 | C14orf18, Cyclin-B1-interacting protein 1, E3 ubiquitin-protein ligase CCNB1IP1, EC:2.3.2.27, HEI10 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC004435 |
| 基因名称 | CCNB1IP1 |
| Gene ID (NCBI) | 57820 |
| RRID | AB_3742873 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9NPC3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin-B1-interacting protein 1 (CCNB1IP1) is an interacting protein of cyclin B1 involved in regulating cell cycle progression, and may be implicated in tumour events (PMID: 12612082). CCNB1IP1 modulates cyclin-B levels and participates in the regulation of cell cycle progression through the G2 phase. Its overexpression causes delayed entry into mitosis (PMID: 12612082; 17297447). Additionally, CCNB1IP1 has been shown to stabilize the MYCN protein, which is a critical factor in neuroblastoma tumorigenesis (PMID: 37461251).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCNB1IP1 antibody 33150-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
