验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| CoIP | See 1 publications below |
产品信息
26270-1-AP targets CCM2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag24233 Product name: Recombinant human CCM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 88-298 aa of BC004903 Sequence: ILHFIDNAKRAHQLPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRVPIHDIAAVSYVRDDAAHLVVLKTAQDPGISPSQSLCAESSRGLSAGSLSESAVGPVEACCLVILAAESKVAAEELCCLLGQVFQVVYTESTIDFLDRAIFDGASTPTHHLSLHSDDSSTKVDIKETYEVEASTFCFPESVDVGGASPHSKTISESELSASATEL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cerebral cavernous malformation 2 |
| 别名 | C7orf22, CCM2, Malcavernin, OSM, PP10187 |
| 计算分子量 | 49 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC004903 |
| 基因名称 | CCM2 |
| Gene ID (NCBI) | 83605 |
| RRID | AB_2880454 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BSQ5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CCM2 antibody 26270-1-AP | Download protocol |
| IHC protocol for CCM2 antibody 26270-1-AP | Download protocol |
| WB protocol for CCM2 antibody 26270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Dual recruitment of two CCM2 molecules to KRIT1 suppresses KLF4 expression.
| ||
Biochem J CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading.
| ||
Endocrinology Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice
| ||
Int Immunol Polymorphic tail of HLA-DQ leads to the heterogeneous IL-10 response of antigen-presenting cells. |
