验证数据展示
经过测试的应用
| Positive WB detected in | human peripheral blood platelets, PMA, LPS and Brefeldin A treated THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12000-1-AP targets CCL5/RANTES in WB, IHC, IF, Neutralization, ELISA, Blocking assay applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, Neutralization, Blocking assay |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2617 Product name: Recombinant human CCL5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-91 aa of BC008600 Sequence: MKVSAAALAVILIATALCAPASASPYSSDTTPCCFAYIARPLPRAHIKEYFYTSGKCSNPAVVFVTRKNRQVCANPEKKWVREYINSLEMS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 5 |
| 别名 | CCL5, C C motif chemokine 5, C-C motif chemokine 5, CCL 5, CCL-5 |
| 计算分子量 | 91 aa, 10 kDa |
| 观测分子量 | 8 kDa |
| GenBank蛋白编号 | BC008600 |
| 基因名称 | CCL5/RANTES |
| Gene ID (NCBI) | 6352 |
| RRID | AB_2877815 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13501 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RANTES (CCL5) belongs to the CC chemokine family and induces leukocyte migration by binding to specific receptors in the G protein-coupled receptor family. RANTES (CCL5) has been shown in vitro to mediate eosinophil, lymphocyte, neutrophil, and monocyte chemotaxis, and it initiates several other proinflammatory events, such as integrin activation, lipid mediator biosynthesis, and degranulation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCL5/RANTES antibody 12000-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater Endogenous Metal Ion-Enriched Immunostimulating Biomimetic Scaffold Improves In Situ Bone Regeneration. | ||
Drug Resist Updat The combination of flaxseed lignans and PD-1/ PD-L1 inhibitor inhibits breast cancer growth via modulating gut microbiome and host immunity | ||
Research (Wash D C) PTBP2-Mediated Alternative Splicing of IRF9 Controls Tumor-Associated Monocyte/Macrophage Chemotaxis and Repolarization in Neuroblastoma Progression | ||
Cell Death Dis Tumor-derived exosomal CCT6A serves as a matchmaker introducing chemokines to tumor-associated macrophages in pancreatic ductal adenocarcinoma | ||
J Immunother Cancer Chordoma recruits and polarizes tumor-associated macrophages via secreting CCL5 to promote malignant progression | ||
Mol Ther Targeting S100A8/A9-NCF1 Axis in Tumor microenvironment to Prevent Tumor Metastasis by Self-assembled Peptide Nanofibers |
