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验证数据展示

  • WB analysis of mouse brain using 19431-1-AP

    WB analysis of mouse brain using 19431-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19431-1-AP (CCDC94 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 19431-1-AP

    IHC staining of human placenta using 19431-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19431-1-AP (CCDC94 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 19431-1-AP

    IHC staining of human placenta using 19431-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19431-1-AP (CCDC94 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of HeLa using 19431-1-AP

    WB analysis of HeLa using 19431-1-AP

    WB result of CCDC94 antibody (19431-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC94 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

CCDC94 Polyclonal antibody

CCDC94 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19431-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CCDC94

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19431-1-AP targets CCDC94 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13764

Product name: Recombinant human CCDC94 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-323 aa of BC000561

Sequence: MSERKVLNKYYPPDFDPSKIPKLKLPKDRQYVVRLMAPFNMRCKTCGEYIYKGKKFNARKETVQNEVYLGLPIFRFYIKCTRCLAEITFKTDPENTDYTMEHGATRNFQAEKLLEEEEKRVQKEREDEELNNPMKVLENRTKDSKLEMEVLENLQELKDLNQRQAHVDFEAMLRQHRLSEEERRRQQQEEDEQETAALLEEARKRRLLEDSDSEDEAAPSPLQPALRPNPTAILDEAPKPKRKVEVWEQSVGSLGSRPPLSRLVVVKKAKADPDCSNGQPQAAPTPGAPQNRKEANPTPLTPGASSLSQLGAYLDSDDSNGSN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 94
别名 CCDC94
计算分子量 323 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC000561
基因名称 CCDC94
Gene ID (NCBI) 55702
RRIDAB_10641994
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BW85
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

实验方案

Product Specific Protocols
WB protocol for CCDC94 antibody 19431-1-APDownload protocol
IHC protocol for CCDC94 antibody 19431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols