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CCDC94 Polyclonal antibody

CCDC94 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 19431-1-AP

Print datasheet

Synonyms

CCDC94

验证数据展示

  • WB analysis of mouse brain using 19431-1-AP

    WB analysis of mouse brain using 19431-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19431-1-AP (CCDC94 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 19431-1-AP

    IHC staining of human placenta using 19431-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19431-1-AP (CCDC94 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 19431-1-AP

    IHC staining of human placenta using 19431-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19431-1-AP (CCDC94 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of HeLa using 19431-1-AP

    WB analysis of HeLa using 19431-1-AP

    WB result of CCDC94 antibody (19431-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC94 transfected HeLa cells.

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19431-1-AP targets CCDC94 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen CCDC94 fusion protein Ag13764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 94
Synonyms CCDC94
Calculated Molecular Weight 323 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC000561
Gene Symbol CCDC94
Gene ID (NCBI) 55702
RRIDAB_10641994
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BW85
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

实验方案

Product Specific Protocols
WB protocol for CCDC94 antibody 19431-1-APDownload protocol
IHC protocol for CCDC94 antibody 19431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols