验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, human testis tissue, mouse colon tissue, Jurkat cells, human testis tissues |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human colon tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CCDC93 Ready-To-Use IHC Kit. CCDC93 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20861-1-AP targets CCDC93 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag14855 Product name: Recombinant human CCDC93 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 282-631 aa of BC028609 Sequence: CSAEIKQIVSEYAEKQSELSAEESPEKLGTSQLHRRKVISLNKQIAQKTKHLEELRASHTSLQARYNEAKKTLTELKTYSEKLDKEQAALEKIESKADPSILQNLRALVAMNENLKSQEQEFKAHCREEMTRLQQEIENLKAERAPRGDEKTLSSGEPPGTLTSAMTHDEDLDRRYNMEKEKLYKIRLLQARRNREIAILHRKIDEVPSRAELIQYQKRFIELYRQISAVHKETKQFFTLYNTLDDKKVYLEKEISLLNSIHENFSQAMASPAARDQFLRQMEQIVEGIKQSRMKMEKKKQENKMRRDQLNDQYLELLEKQRLYFKTVKEFKEEGRKNEMLLSKVKAKAS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 93 |
| 别名 | CCDC93 |
| 计算分子量 | 631 aa, 73 kDa |
| 观测分子量 | 73 kDa |
| GenBank蛋白编号 | BC028609 |
| 基因名称 | CCDC93 |
| Gene ID (NCBI) | 54520 |
| RRID | AB_10696446 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q567U6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCDC93 antibody 20861-1-AP | Download protocol |
| IP protocol for CCDC93 antibody 20861-1-AP | Download protocol |
| WB protocol for CCDC93 antibody 20861-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. | ||
J Extracell Vesicles Genome-Wide CRISPR/Cas9 Screening Identifies the COMMANDER Recycling Complex as a Key Player in EV Uptake.
| ||
Nat Commun CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. | ||
Sci Immunol Celastrol suppresses humoral immune responses and autoimmunity by targeting the COMMD3/8 complex | ||
Mol Metab Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion. |
