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Cyclon/CCDC86 Polyclonal antibody

Cyclon/CCDC86 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14947-1-AP

Print datasheet

Synonyms

CCDC86, CYCLON, Cyclon/CCDC86

验证数据展示

  • WB analysis of mouse stomach using 14947-1-AP

    WB analysis of mouse stomach using 14947-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 14947-1-AP

    IF Staining of HepG2 using 14947-1-AP

    Immunofluorescent analysis of HepG2 cells using 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse stomach tissue, HepG2 cells, human heart tissue, human liver tissue, human stomach tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14947-1-AP targets Cyclon/CCDC86 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Cyclon/CCDC86 fusion protein Ag6754 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 86
Synonyms CCDC86, CYCLON, Cyclon/CCDC86
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC001378
Gene Symbol CCDC86
Gene ID (NCBI) 79080
RRIDAB_2072318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6F5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Cyclon/CCDC86 antibody 14947-1-APDownload protocol
IF protocol for Cyclon/CCDC86 antibody 14947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols