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验证数据展示

  • IHC staining of human liver using 18282-1-AP (same clone as 18282-1-PBS)

    IHC staining of human liver using 18282-1-AP (same clone as 18282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18282-1-AP (CCDC85B antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 18282-1-AP (same clone as 18282-1-PBS)

    IHC staining of human liver using 18282-1-AP (same clone as 18282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18282-1-AP (CCDC85B antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 18282-1-AP (same clone as 18282-1-PBS)

    WB analysis of mouse heart using 18282-1-AP (same clone as 18282-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18282-1-AP (CCDC85B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 18282-1-AP (same clone as 18282-1-PBS)

    IP experiment of mouse heart using 18282-1-AP (same clone as 18282-1-PBS)

    IP result of anti-CCDC85B (IP:18282-1-AP, 4ug; Detection:18282-1-AP 1:300) with mouse heart tissue lysate 3600ug. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18282-1-AP (same clone as 18282-1-PBS)

    IF Staining of HeLa using 18282-1-AP (same clone as 18282-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18282-1-AP (CCDC85B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

CCDC85B Polyclonal antibody, PBS Only

CCDC85B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18282-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18282-1-PBS targets CCDC85B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13129

Product name: Recombinant human CCDC85B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC008796

Sequence: MEAEAGGLEELTDEEMAALGKEELVRRLRREEAARLAALVQRGRLMQEVNRQLQGHLGEIRELKQLNRRLQAENRELRDLCCFLDSERQRGRRAARQWQLFGTQASRAVREDLGGCWQKLAELEGRQEELLRENLALKELCLALGEEWGPRGGPSGAGGSGAGPAPELALPPCGPRDLGDGSSSTGSVGSPDQLPLACSPDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 85B
别名
计算分子量 22 kDa
观测分子量 24-28 kDa
GenBank蛋白编号BC008796
基因名称 CCDC85B
Gene ID (NCBI) 11007
RRIDAB_2878527
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15834
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Coiled-coil domain-containing protein 85B (CCDC85B), also named as DIPA, is 202 amino acid protein, which belongs to the CCDC85 family. CCDC85B localizes in the nucleus and cytoplasm. CCDC85B is widely expressed in many tissues and functions as a transcriptional repressor. CCDC85B may inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. CCDC85B may play a role in adipocyte differentiation.

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