验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
27194-1-AP targets CCDC8 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CCDC8 fusion protein Ag25794 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 8 |
| 别名 | CCDC8 |
| 观测分子量 | 90 kDa, 59 kDa |
| GenBank蛋白编号 | BC025243 |
| 基因名称 | CCDC8 |
| Gene ID (NCBI) | 83987 |
| RRID | AB_2880795 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H0W5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Coiled-coil domain-containing protein 8 (CCDC8) is a 538-amino acid protein with the molecular mass of 59kD and 90kD cause of the posttranslational modifications including amidation, glycosylation, phosphorylation, and myristalation (PMID: 21737058). CCDC8 has 2 coiled-coil domains, and the proteins containing this domain are involved in diverse biological processes, such as the regulation of gene expression, cell division, and membrane fusion. CCDC8 is evolutionarily conserved, and CCDC8 mutation leads to the development of 3M syndrome in humans, a primordial growth disorder, by interacting with CUL7, OBSL1, and P53. Low-level or no expression of CCDC8 was shown to be closely related to the development of some tumors, such as renal cell carcinoma, multiple myeloma, breast cancer, and prostate cancer (PMID: 21737058, 27342910).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC8 antibody 27194-1-AP | Download protocol |
| IHC protocol for CCDC8 antibody 27194-1-AP | Download protocol |
| IP protocol for CCDC8 antibody 27194-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci Ablation of CCDC8 provides cardioprotection against cardiomyocyte apoptosis via TNF signaling pathway in myocardial ischemia reperfusion injury
| ||
J Cancer Prognostic Significance of CCDC8 in Bladder Cancer: Insights from Bioinformatics and Immunohistochemical Analysis |