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验证数据展示

  • WB analysis using 28152-1-AP

    WB analysis using 28152-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28152-1-AP (CCDC71 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28152-1-AP

    IHC staining of human breast cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 28152-1-AP

    IHC staining of human breast cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCDC71 Polyclonal antibody

CCDC71 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28152-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

CCDC71, FLJ12800, FLJ13629

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28152-1-AP targets CCDC71 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 CCDC71 fusion protein Ag27446 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 71
别名 CCDC71, FLJ12800, FLJ13629
计算分子量 467 aa, 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC035516
基因名称 CCDC71
Gene ID (NCBI) 64925
RRIDAB_2881075
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IV32
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC71 antibody 28152-1-APDownload protocol
IHC protocol for CCDC71 antibody 28152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols