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验证数据展示

  • WB analysis using 31457-1-AP

    WB analysis using 31457-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31457-1-AP (CCDC69 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 31457-1-AP

    WB analysis of U2OS using 31457-1-AP

    WB result of CCDC69 antibody (31457-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC69 transfected U2OS cells.

  • Featured Product
  • KD/KO验证

CCDC69 Polyclonal antibody

CCDC69 Polyclonal Antibody for WB, ELISA
Cat No. 31457-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

FLJ13705

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, MDA-MB-231 cells, RT-4 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31457-1-AP targets CCDC69 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CCDC69 fusion protein Ag34858 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 69
别名 FLJ13705
观测分子量43-45 kDa
GenBank蛋白编号BC016647
基因名称 CCDC69
Gene ID (NCBI) 26112
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDA6NI79
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC69 (Coiled-coil domain-containing protein 69), may act as a scaffold to regulate the recruitment and assembly of spindle midzone components, which is required for the localization of AURKB and PLK1 to the spindle midzone (PMID: 20962590). It is highly expressed in duodenum, esophagus, pancreas, prostate, salivary gland, thymus and urinary bladder. The molecular weight of CCDC69 is 34 kDa, and it has been reported that the protein has a molecular weight of 43kDa (PMID: 20962590).

实验方案

Product Specific Protocols
WB protocol for CCDC69 antibody 31457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols