验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, SH-SY5Y cells |
| Positive IHC detected in | human testis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
26301-1-AP targets CCDC68 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag23622 Product name: Recombinant human CCDC68 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-335 aa of BC029508 Sequence: MTTVTVTTEIPPRDKMEDNSALYESTSAHIIEETEYVKKIRTTLQKIRTQMFKDEIRHDSTNHKLDAKHCGNLQQGSDSEMDPSCCSLDLLMKKIKGKDLQLLEMNKENEVLKIKLQASREAGAAALRNVAQRLFENYQTQSEEVRKKQEDSKQLLQVNKLEKEQKLKQHVENLNQVAEKLEEKHSQITELENLVQRMEKEKRTLLERKLSLENKLLQLKSSATYGKSCQDLQREISILQEQISHLQFVIHSQHQNLRSVIQEMEGLKNNLKEQDKRIENLREKVNILEAQNKELKTQVALSSETPRTKVSKAVSTSELKTEGVSPYLMLIRLRK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 68 |
| 别名 | CCDC68, CTCL associated antigen se57 1, SE57 1 |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC029508 |
| 基因名称 | CCDC68 |
| Gene ID (NCBI) | 80323 |
| RRID | AB_2880470 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H2F9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Coiled-coil domain containing 68 (CCDC68) demonstrates a tumor-suppressive role in pancreatic ductal adenocarcinoma. (PMID: 25381825)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC68 antibody 26301-1-AP | Download protocol |
| IHC protocol for CCDC68 antibody 26301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages. | ||
Adv Sci (Weinh) CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC |
