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验证数据展示

  • WB analysis using 22770-1-AP

    WB analysis using 22770-1-AP

    MCF-7 lysates were subjected to SDS PAGE followed by western blot with 22770-1-AP (CCDC64B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22770-1-AP

    IHC staining of human breast cancer using 22770-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22770-1-AP (CCDC64B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 22770-1-AP

    IHC staining of human breast cancer using 22770-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22770-1-AP (CCDC64B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCDC64B Polyclonal antibody

CCDC64B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22770-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Bicaudal D related protein 2, BICD related protein 2, BICDR 2, BICDR2, CCDC64B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22770-1-AP targets CCDC64B in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 CCDC64B fusion protein Ag18720 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 64B
别名 Bicaudal D related protein 2, BICD related protein 2, BICDR 2, BICDR2, CCDC64B
计算分子量 508 aa, 57 kDa
观测分子量57 kDa
GenBank蛋白编号BC128602
基因名称 CCDC64B
Gene ID (NCBI) 146439
RRIDAB_2918078
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA1A5D9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC64B, also named as BICDL2 and BICDR2, belongs to BICD family. It was officially named by Schlager et al (2010) in a sequence homology search for new mammalian BICD-like proteins. At present, there is still little study on this protein, which may involve the vesicle transport along microtubule, and Golgi to secretory granule transport. BICDR2 has 2 isoforms with the molecular mass of 57 and 34 kDa (PMID:20360680, 20485297).

实验方案

Product Specific Protocols
WB protocol for CCDC64B antibody 22770-1-APDownload protocol
IHC protocol for CCDC64B antibody 22770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols