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验证数据展示

  • IHC staining of human colon cancer using 20427-1-AP

    IHC staining of human colon cancer using 20427-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 20427-1-AP

    IHC staining of human colon cancer using 20427-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20427-1-AP (CCDC56 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 20427-1-AP

    IF Staining of U2OS using 20427-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCDC56 antibody (20427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of A431 using 20427-1-AP

    IF Staining of A431 using 20427-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC56 antibody (20427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CCDC56/COA3 Polyclonal antibody

CCDC56/COA3 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 20427-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

CCDC56, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 12 kDa, HSPC009, Cytochrome c oxidase assembly factor 3 homolog, mitochondrial, Coiled-coil domain-containing protein 56

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

20427-1-AP targets CCDC56/COA3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CCDC56/COA3 fusion protein Ag14260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 56
别名 CCDC56, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 12 kDa, HSPC009, Cytochrome c oxidase assembly factor 3 homolog, mitochondrial, Coiled-coil domain-containing protein 56
计算分子量 106 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC002698
基因名称 CCDC56
Gene ID (NCBI) 28958
RRIDAB_10695182
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2R0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human COX assembly factor 3 (COA3), also known as CCDC56 (coiled-coil domain-containing protein 56), is a mitochondrial transmembrane protein responsible for cytochrome c oxidase (COX) protein complex assembly. CCDC56/COA3 is located on chromosome 17 (17q.21.31) and encodes an 11.7-kDa protein with predicted transmembrane and coiled-coil domains (PMID: 22610097). CCDC56/COA3 is overexpressed at both mRNA and protein levels in human NSCLC cells, mainly as a result of decreased miR-338-3p level (PMID: 36119836).

实验方案

Product Specific Protocols
IHC protocol for CCDC56/COA3 antibody 20427-1-APDownload protocol
IF protocol for CCDC56/COA3 antibody 20427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Am J Cancer Res

COA3 overexpression promotes non-small cell lung cancer metastasis by reprogramming glucose metabolism

Authors - Hongwei Lin
View Article