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CCDC55 Polyclonal antibody

CCDC55 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21360-1-AP

Print datasheet

Synonyms

CCDC55, HSPC095

验证数据展示

  • IHC staining of human brain using 21360-1-AP

    IHC staining of human brain using 21360-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21360-1-AP (CCDC55 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 21360-1-AP

    IHC staining of human brain using 21360-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21360-1-AP (CCDC55 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of COLO 320 using 21360-1-AP

    WB analysis of COLO 320 using 21360-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21360-1-AP (CCDC55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21360-1-AP is CCDC55 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitypig
Immunogen CCDC55 fusion protein Ag15811 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 55
Synonyms CCDC55, HSPC095
Calculated Molecular Weight 558 aa, 66 kDa
Observed Molecular Weight 66 kDa 70 kDa
GenBank Accession NumberBC040118
Gene Symbol CCDC55
Gene ID (NCBI) 84081
RRIDAB_2878847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H0G5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC55, also named as NSRP1 and NSRP70, is a conserved coiled-coil domain containing protein. It is a novel regulator of cell migration. This antibody detects 66 kDa (native form) and 70 kDa (phospho form).

实验方案

Product Specific Protocols
WB protocol for CCDC55 antibody 21360-1-APDownload protocol
IHC protocol for CCDC55 antibody 21360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Int J Mol Sci

Comprehensive Atlas of Alternative Splicing Reveals NSRP1 Promoting Adipogenesis through CCDC18

Authors - Lei Liu
  • KD Validated
View Article