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验证数据展示

  • IHC staining of human placenta using 24445-1-AP

    IHC staining of human placenta using 24445-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24445-1-AP (CCDC53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 24445-1-AP

    IHC staining of human placenta using 24445-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24445-1-AP (CCDC53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCDC53 Polyclonal antibody

CCDC53 Polyclonal Antibody for IHC, ELISA
Cat No. 24445-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA and More (1)

别名

CCDC53, CGI 116, WASH complex subunit CCDC53, WASHC3, x0009

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman placenta tissue, human breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

24445-1-AP targets CCDC53 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag19831

Product name: Recombinant human CCDC53 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-194 aa of BC010889

Sequence: MDEDGLPLMGSGIDLTKVPAIQQKRTVAFLNQFVVHTVQFLNRFSTVCEEKLADLSLRIQQIETTLNILDAKLSSIPGLDDVTVEVSPLNVTSVTNGAHPEATSEQPQQSSTQDSGLQESEVSAENILTVAKDPRYARYLKMVQVGVPVMAIRNKMISEGLDPDLLERPDAPVPDGESEKTVEESSDSESSFSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 53
别名 CCDC53, CGI 116, WASH complex subunit CCDC53, WASHC3, x0009
计算分子量 194 aa, 21 kDa
GenBank蛋白编号BC010889
基因名称 CCDC53
Gene ID (NCBI) 51019
RRIDAB_2879551
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3C0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CCDC53 antibody 24445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Architecture of the human interactome defines protein communities and disease networks.

Authors - Edward L Huttlin
View Article
humanWB

Nat Commun

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Authors - Yunkai Zhu
View Article