验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, HeLa cells, mouse thymus tissue, Jurkat cells, MCF-7 cells |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14807-1-AP targets CCDC28A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CCDC28A fusion protein Ag6529 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | coiled-coil domain containing 28A |
别名 | C6orf80, CC28A, CCRL1AP, Coiled-coil domain-containing protein 28A |
计算分子量 | 30 kDa |
观测分子量 | 70 kDa |
GenBank蛋白编号 | BC000758 |
基因名称 | CCDC28A |
Gene ID (NCBI) | 25901 |
RRID | AB_2072069 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8IWP9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Coiled-coil domain-containing protein 28A (CCDC28A) is a translocation partner of nucleoporin 98 (NUP98) in acute leukemias, resulting in the creation of fusion genes and the expression of chimeric proteins. These fusion proteins, such as NUP98-CCDC28A, have been shown to promote myeloproliferative neoplasms (PMID: 22058212). Additionally, CCDC28A is essential for head-tail coupling stability, thereby ensuring sperm motility (PMID: 39500989).
实验方案
Product Specific Protocols | |
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WB protocol for CCDC28A antibody 14807-1-AP | Download protocol |
IF protocol for CCDC28A antibody 14807-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |