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验证数据展示

  • WB analysis using 14807-1-AP

    WB analysis using 14807-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14807-1-AP

    IF Staining of MCF-7 using 14807-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CCDC28A antibody (14807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

CCDC28A Polyclonal antibody

CCDC28A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14807-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

C6orf80, CC28A, CCRL1AP, Coiled-coil domain-containing protein 28A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, HeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14807-1-AP targets CCDC28A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6529

Product name: Recombinant human CCDC28A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-162 aa of BC000758

Sequence: MPKKNAIPVSKSTGFSNPASQSTSQRPKLKRVMKEKTKPQGGEGKGAQSTPIQHSFLTDVSDVQEMERGLLSLLNDFHSGKLQAFGNECSIEQMEHVRGMQEKLARLNLELYGELEELPEDKRKTASDSNLDRLLSDLEELNSSIQKLHLADAQDVPNTSAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 28A
别名 C6orf80, CC28A, CCRL1AP, Coiled-coil domain-containing protein 28A
计算分子量 30 kDa
观测分子量70 kDa
GenBank蛋白编号BC000758
基因名称 CCDC28A
Gene ID (NCBI) 25901
RRIDAB_2072069
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWP9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coiled-coil domain-containing protein 28A (CCDC28A) is a translocation partner of nucleoporin 98 (NUP98) in acute leukemias, resulting in the creation of fusion genes and the expression of chimeric proteins. These fusion proteins, such as NUP98-CCDC28A, have been shown to promote myeloproliferative neoplasms (PMID: 22058212). Additionally, CCDC28A is essential for head-tail coupling stability, thereby ensuring sperm motility (PMID: 39500989).

实验方案

Product Specific Protocols
IF protocol for CCDC28A antibody 14807-1-APDownload protocol
WB protocol for CCDC28A antibody 14807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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