验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32228-1-AP targets CCDC157 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | CCDC157 fusion protein Ag37002 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 157 |
| 别名 | KIAA1656, Coiled-coil domain-containing protein 157 |
| 计算分子量 | 84 kDa, 752 aa |
| 观测分子量 | 85 kDa |
| GenBank蛋白编号 | BC092419 |
| 基因名称 | CCDC157 |
| Gene ID (NCBI) | 550631 |
| RRID | AB_3670225 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q569K6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCDC157 is a member of the coiled‐coil domain‐containing (CCDC) family proteins, wich have a conserved coiled‐coil motif that is responsible for molecular recognition and protein refolding. CCDC157 is highly expressed in the testes from Drosophila to mammals. Bioinformatics database analysis suggests that CCDC157 expression is significantly decreased in NOA patients (PMID: 30741974). An in vitro study has reported that CCDC157 is localized on Golgi membranes along the secretory pathway and interacts with resident proteins of ER‐Golgi membranes (PMID: 31488582).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC157 antibody 32228-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |