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验证数据展示

  • WB analysis using 21390-1-AP

    WB analysis using 21390-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 21390-1-AP

    IHC staining of human prostate hyperplasia using 21390-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 21390-1-AP (CCDC153 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate hyperplasia using 21390-1-AP

    IHC staining of human prostate hyperplasia using 21390-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 21390-1-AP (CCDC153 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21390-1-AP

    IF Staining of HepG2 using 21390-1-AP

    Immunofluorescent analysis of HepG2 cells, using CCDC153 antibody 21390-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • WB analysis of COLO 320 using 21390-1-AP

    WB analysis of COLO 320 using 21390-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

CCDC153 Polyclonal antibody

CCDC153 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21390-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Dynein regulatory complex subunit 12 homolog, Dynein regulatory complex protein 12, DRC12, Coiled-coil domain-containing protein 153

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells, L02 cells, human brain tissue, HeLa cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21390-1-AP targets CCDC153 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16044

Product name: Recombinant human CCDC153 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-124 aa of BC101443

Sequence: MSRQCHALQEDMQTHSKQLEEEVKGLRGQLEACQREAAAAREEAEQALGERDQALAQLRAHMADMEAKYEEILHDSLDRLLAKLRAIKQQWDGAALRLHARHKEQQRQFGLTPPGSLRPPAPSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 153
别名 Dynein regulatory complex subunit 12 homolog, Dynein regulatory complex protein 12, DRC12, Coiled-coil domain-containing protein 153
计算分子量 210 aa, 24 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC101443
基因名称 CCDC153
Gene ID (NCBI) 283152
RRIDAB_10858621
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ494R4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC153's properties have yet to be elucidated. However, research suggests that it may be a neuronal subtype marker (PMID: 28166221). It has a molecular weight of 24 KDa and is often observed on a Western blot as a dimer.

实验方案

Product Specific Protocols
IF protocol for CCDC153 antibody 21390-1-APDownload protocol
IHC protocol for CCDC153 antibody 21390-1-APDownload protocol
WB protocol for CCDC153 antibody 21390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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