• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 25138-1-AP

    WB analysis of HeLa using 25138-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25138-1-AP (CCDC12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25138-1-AP

    IP experiment of HeLa using 25138-1-AP

    IP result of anti-CCDC12 (IP:25138-1-AP, 4ug; Detection:25138-1-AP 1:600) with HeLa cells lysate 2560ug.

  • IHC staining of human ovary tumor using 25138-1-AP

    IHC staining of human ovary tumor using 25138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25138-1-AP (CCDC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCDC12 antibody included
  • IF Staining of HeLa using 25138-1-AP

    IF Staining of HeLa using 25138-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCDC12 antibody (25138-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IHC staining of human ovary tumor using 25138-1-AP

    IHC staining of human ovary tumor using 25138-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25138-1-AP (CCDC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCDC12 antibody included

CCDC12 Polyclonal antibody

CCDC12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25138-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CCDC12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CCDC12 Ready-To-Use IHC Kit. CCDC12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25138-1-AP targets CCDC12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 CCDC12 fusion protein Ag18223 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 12
别名 CCDC12
计算分子量 166 aa, 19 kDa
观测分子量 18-25 kDa
GenBank蛋白编号BC020830
基因名称 CCDC12
Gene ID (NCBI) 151903
RRIDAB_2879919
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WUD4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC12 is a 166aa protein with MW 18-25 kDa (with some modification).

实验方案

Product Specific Protocols
WB protocol for CCDC12 antibody 25138-1-APDownload protocol
IHC protocol for CCDC12 antibody 25138-1-APDownload protocol
IF protocol for CCDC12 antibody 25138-1-APDownload protocol
IP protocol for CCDC12 antibody 25138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols