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CCDC105 Polyclonal antibody

CCDC105 Polyclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24026-1-AP

Print datasheet

Synonyms

CCDC105

验证数据展示

  • WB analysis of DU 145 using 24026-1-AP

    WB analysis of DU 145 using 24026-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24026-1-AP

    IHC staining of human testis using 24026-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 24026-1-AP

    IHC staining of human testis using 24026-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 24026-1-AP

    IF Staining of HeLa using 24026-1-AP

    Immunofluorescent analysis of HeLa cells using 24026-1-AP (CCDC105 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 24026-1-AP

    IF Staining of mouse testis using 24026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 24026-1-AP

    IF Staining of mouse testis using 24026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inDU 145 cells, HeLa cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24026-1-AP targets CCDC105 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC,IF-P, IHC, WB, ELISA
Tested Reactivity human
Immunogen CCDC105 fusion protein Ag21208 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 105
Synonyms CCDC105
Calculated Molecular Weight 499 aa, 57 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC035678
Gene Symbol CCDC105
Gene ID (NCBI) 126402
RRIDAB_2879406
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8IYK2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC105 antibody 24026-1-APDownload protocol
IHC protocol for CCDC105 antibody 24026-1-APDownload protocol
IF protocol for CCDC105 antibody 24026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols