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验证数据展示

  • WB analysis of DU 145 using 24026-1-AP

    WB analysis of DU 145 using 24026-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24026-1-AP

    IHC staining of human testis using 24026-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 24026-1-AP

    IHC staining of human testis using 24026-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of mouse testis using 24026-1-AP

    IF Staining of mouse testis using 24026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 24026-1-AP

    IF Staining of mouse testis using 24026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CCDC105 Polyclonal antibody

CCDC105 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 24026-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF-P, ELISA

别名

Coiled-coil domain-containing protein 105, FLJ40365, Tektin-like protein 1, TEKTL1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDU 145 cells, HeLa cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24026-1-AP targets CCDC105 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原

CatNo: Ag21208

Product name: Recombinant human CCDC105 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 151-499 aa of BC035678

Sequence: VRQLLRQREVTDHRLSEVRKGLLINQQSVKLRGYRPKSEKVPDKADSMLTWEKEELKSMKRKMERDMEKSEVLLKTLASCRDTLNFCFKERLQAVDLMNQPLDKVLEQARRHSWVNLSRAPTPRTQGQKTPPPDPVGTYNPACALALNEAKRLLVESKDTLVEMAKNEVDVREQQLQISDRVCASLAQKASETLELKERLNMTLGLMRGTILRCTKYNQELYTTHGLIKGPLSKVHLETAEKLDRPLVRMYQRHVGTQLPEAARLAQGTDKLQCHITYLEKNLEELLATHKNLTWGLNCKNIGHEVDGNVVRLRLRQRQPHVCYEQAQRLVKDWDPRTPPPRSKSSADP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 105
别名 Coiled-coil domain-containing protein 105, FLJ40365, Tektin-like protein 1, TEKTL1
计算分子量 499 aa, 57 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC035678
基因名称 CCDC105
Gene ID (NCBI) 126402
RRIDAB_2879406
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8IYK2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CCDC105 antibody 24026-1-APDownload protocol
IHC protocol for CCDC105 antibody 24026-1-APDownload protocol
WB protocol for CCDC105 antibody 24026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

PLoS Biol

The core outer junction protein CFAP77 connects A- and B-tubules within doublet microtubules of cilia and flagella

Authors - Lan Xia
View Article
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