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验证数据展示

  • WB analysis of mouse lung using 24276-1-AP

    WB analysis of mouse lung using 24276-1-AP

    mouse lung tissues were subjected to SDS PAGE followed by western blot with 24276-1-AP (CFAP36 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 24276-1-AP

    IF Staining of A431 using 24276-1-AP

    Immunofluorescent analysis of A431 cells using 24276-1-AP (CCDC104 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of mouse stomach using 24276-1-AP

    IHC staining of mouse stomach using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 24276-1-AP

    IHC staining of mouse stomach using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat lung using 24276-1-AP

    IHC staining of rat lung using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CFAP36 Polyclonal antibody

CFAP36 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24276-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CCDC104, Cilia- and flagella-associated protein 36, coiled coil domain containing 104, Coiled-coil domain-containing protein 104, UNQ163/PRO189

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, HEK-293 cells
Positive IHC detected inmouse stomach tissue, human testis tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24276-1-AP targets CFAP36 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17996

Product name: Recombinant human CCDC104 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-342 aa of BC010011

Sequence: MAAEEEDEVEWVVESIAGFLRGPDWSIPILDFVEQKCEVFDDEEESKLTYTEIHQEYKELVEKLLEGYLKEIGINEDQFQEACTSPLAKTHTSQAILQPVLAAEDFTIFKAMMVQKNIEMQLQAIRIIQERNGVLPDCLTDGSDVVSDLEHEEMKILREVLRKSKEEYDQEEERKRKKQLSEAKTEEPTVHSSEAAIMNNSQGDGEHFAHPPSEVKMHFANQSIEPLGRKVERSETSSLPQKGLKFPGLEHASIEGPIANLSVLGTEELRQREHYLKQKRDKLMSMRKDMRTKQIQNMEQKGKPTGEVEEMTEKPEMTAEEKQTLLKRRLLAEKLKEEVINK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 104
别名 CCDC104, Cilia- and flagella-associated protein 36, coiled coil domain containing 104, Coiled-coil domain-containing protein 104, UNQ163/PRO189
计算分子量 342 aa, 39 kDa
观测分子量 42 kDa
GenBank蛋白编号BC010011
基因名称 CCDC104
Gene ID (NCBI) 112942
RRIDAB_2879466
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96G28
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CFAP36 (Cilia- and flagella-associated protein 36), also known as CCDC104(Coiled-coil domain-containing protein 104), whose function is unknown, is expressed in various human tissues, including the brain, and is localized mainly to the nucleus, but is also found in the cytoplasm(PMID:19680650). It may act as an effector for ARL3. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CFAP36 antibody 24276-1-APDownload protocol
IHC protocol for CFAP36 antibody 24276-1-APDownload protocol
IF protocol for CFAP36 antibody 24276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell

A conserved mechanism for the retrieval of polyubiquitinated proteins from cilia

Authors - Sven M Lange
View Article