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验证数据展示

  • WB analysis of mouse lung using 24276-1-AP

    WB analysis of mouse lung using 24276-1-AP

    mouse lung tissues were subjected to SDS PAGE followed by western blot with 24276-1-AP (CCDC104 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24276-1-AP

    IHC staining of human testis using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50.

  • IHC staining of human testis using 24276-1-AP

    IHC staining of human testis using 24276-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50.

  • IF Staining of A431 using 24276-1-AP

    IF Staining of A431 using 24276-1-AP

    Immunofluorescent analysis of A431 cells using 24276-1-AP (CCDC104 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

CCDC104 Polyclonal antibody

CCDC104 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24276-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CCDC104

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, HEK-293 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24276-1-AP targets CCDC104 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CCDC104 fusion protein Ag17996 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 104
别名 CCDC104
计算分子量 342 aa, 39 kDa
观测分子量 42 kDa
GenBank蛋白编号BC010011
基因名称 CCDC104
Gene ID (NCBI) 112942
RRIDAB_2879466
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96G28
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The CCDC104(Coiled-coil domain-containing protein 104) protein, whose function is unknown, is expressed in various human tissues, including the brain, and is localized mainly to the nucleus, but is also found in the cytoplasm(PMID:19680650). It may act as an effector for ARL3. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CCDC104 antibody 24276-1-APDownload protocol
IHC protocol for CCDC104 antibody 24276-1-APDownload protocol
IF protocol for CCDC104 antibody 24276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols