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验证数据展示

  • IHC staining of human tonsillitis using 60046-1-Ig

    IHC staining of human tonsillitis using 60046-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60046-1-Ig (ACKR2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 60046-1-Ig

    IHC staining of human tonsillitis using 60046-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60046-1-Ig (ACKR2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 60046-1-Ig

    IF Staining of human tonsillitis using 60046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ACKR2 antibody (60046-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60046-1-Ig

    IF Staining of human tonsillitis using 60046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ACKR2 antibody (60046-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

ACKR2 Monoclonal antibody

ACKR2 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 60046-1-Ig

产品说明书

CloneNo. 1A3A2
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, IF-P, ELISA and More (1)

别名

CCBP2, 1A3A2, Atypical chemokine receptor 2, C C chemokine receptor D6, C-C chemokine receptor D6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60046-1-Ig targets ACKR2 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 ACKR2 fusion protein Ag1941 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chemokine binding protein 2
别名 CCBP2, 1A3A2, Atypical chemokine receptor 2, C C chemokine receptor D6, C-C chemokine receptor D6
计算分子量 43 kDa
GenBank蛋白编号BC008816
基因名称 ACKR2
Gene ID (NCBI) 1238
RRIDAB_2071883
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00590
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACKR2, also named as CCBP2 or chemokine receptor D6, is a heptahelical membrane protein expressed by lymphatic endothelial cells, binding with high affinity to multiple proinflammatory CC chemokines.

实验方案

Product Specific Protocols
IHC protocol for ACKR2 antibody 60046-1-IgDownload protocol
IF protocol for ACKR2 antibody 60046-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Int Immunopharmacol

-RAMP3 promotes hepatocellular carcinoma tumor cell-mediated CCL2 degradation by supporting membrane distribution of ACKR2

Authors - Lan Yang
View Article