验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human placenta tissue, mouse kidney tissue, SH-SY5Y cells |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20774-1-AP targets CC2D1B in WB, IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag14767 Product name: Recombinant human CC2D1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 468-767 aa of BC007912 Sequence: VAATLAAAEKLASAEDSAPADKDEDEGEPPAQAPVAKKPARPTVPSSQRLPEPRASSSKESPSPSVREQLALLEARKLQYQRAALQAKRSQDLEQAKAYLRVAKWLEAQIIQARSGRPVDLSKVPSPLTDEEGDFILIHHEDLRLSQKAEEVYAQLQKMLLEQQEKCLLFSKQFMHQGNVAETTRFEKLAQDRKKQLEILQLAQAQGLDPPTHHFELKTFQTVRIFSELNSTEMHLIIVRGMNLPAPPGVTPDDLDAFVRFEFHYPNSDQAQKSKTAVVKNTNSPEFDQLFKLNINRNHR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coiled-coil and C2 domain containing 1B |
| 别名 | CC2D1B, Freud-2, KIAA1836, LGD1 |
| 计算分子量 | 858 aa, 94 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC007912 |
| 基因名称 | CC2D1B |
| Gene ID (NCBI) | 200014 |
| RRID | AB_10693627 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T0F9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CC2D1B/Freud2 and CC2D1A/Freud1 are two homologs belonging to the evolutionary conserved Lgd protein family that is involved in endosomal trafficking. CC2D1B has been identified as the novel repressor of postsynaptic 5-HT1A expression. The predicted molecular weight of CC2D1B is 89 kDa, but the observed molecular weight is around 120 kDa, which may be caused by post-translational modification. This antibody is specific to CC2D1B, which has been confirmed by siRNA test. (19423080 , 2672064)
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for CC2D1B antibody 20774-1-AP | Download protocol |
| WB protocol for CC2D1B antibody 20774-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling.
| ||
Front Mol Neurosci Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System.
| ||
Biol Pharm Bull Icariin regulates EMT and stem cell-like character in breast cancer through modulating lncRNA NEAT1/TGFβ/SMAD2 signaling pathway | ||
Nat Commun Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef |
