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验证数据展示

  • WB analysis using 21179-1-AP (same clone as 21179-1-PBS)

    WB analysis using 21179-1-AP (same clone as 21179-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21179-1-AP (CBLL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 21179-1-AP (same clone as 21179-1-PBS)

    IHC staining of human colon cancer using 21179-1-AP (same clone as 21179-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CBLL1 antibody included
  • IF Staining of HepG2 using 21179-1-AP (same clone as 21179-1-PBS)

    IF Staining of HepG2 using 21179-1-AP (same clone as 21179-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBLL1 antibody (21179-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CBLL1 Polyclonal antibody, PBS Only

CBLL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21179-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CBLL1 Ready-To-Use IHC Kit. CBLL1 primary antibody included.

产品信息

21179-1-PBS targets CBLL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15463

Product name: Recombinant human CBLL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-227 aa of BC027460

Sequence: TDNELQGTNSSGSLGGLDVRRRIPIKLISKQANKAKPAPRTQRTINRMPAKAPPGDEEGFDYNEEERYDCKGGELFANQRRFPGHLFWDFQINILGEKDDTPVHFCDKCGLPIKIYGRMIPCKHVFCYDCAILHEKKGDKMCPGCSDPVQRIEQCTRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRAGKPVTRASLENVHPPIAPPPTEIPERFIMPPDKHHMS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
别名
计算分子量 491 aa, 55 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC027460
基因名称 CBLL1
Gene ID (NCBI) 79872
RRIDAB_10732814
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ75N03
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cbl proto‐oncogene E3 ubiquitin protein ligase‐like 1, RNF188, Hakai (CBLL1) is an evolutionarily conserved E3 ubiquitin ligase containing a RING‐finger domain. CBLL1 contains a typical RING‐finger, short pTyr‐binding domain, and proline‐rich domain. CBLL1 expression is upregulated in human colon and gastric cancer tissues, and CBLL1 has been reported to induce anchorage‐dependent cell growth. CBLL1 immunostaining was observed in both the nuclei and cytoplasm of cancer cells. (PMID: 31124298, PMID: 31646569, PMID: 21191016)

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