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验证数据展示

  • WB analysis using 66067-1-Ig

    WB analysis using 66067-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66067-1-Ig

    WB analysis of HeLa using 66067-1-Ig

    WB result of Caveolin-1 antibody (66067-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells.

  • IHC staining of human ovary tumor using 66067-1-Ig

    IHC staining of human ovary tumor using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human breast cancer using 66067-1-Ig

    IHC staining of human breast cancer using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human spleen using 66067-1-Ig

    IHC staining of human spleen using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 66067-1-Ig

    IHC staining of human appendicitis using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 66067-1-Ig

    IHC staining of human liver using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lung using 66067-1-Ig

    IHC staining of human lung using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 66067-1-Ig

    IHC staining of human tonsillitis using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 66067-1-Ig

    IHC staining of human tonsillitis using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human ovary cancer using 66067-1-Ig

    IHC staining of human ovary cancer using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human breast cancer using 66067-1-Ig

    IHC staining of human breast cancer using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human breast cancer using 66067-1-Ig

    IHC staining of human breast cancer using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human stomach cancer using 66067-1-Ig

    IHC staining of human stomach cancer using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human placenta using 66067-1-Ig

    IHC staining of human placenta using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human ovary cancer using 66067-1-Ig

    IHC staining of human ovary cancer using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human tonsillitis using 66067-1-Ig

    IHC staining of human tonsillitis using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human tonsillitis using 66067-1-Ig

    IHC staining of human tonsillitis using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human lung using 66067-1-Ig

    IHC staining of human lung using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human liver using 66067-1-Ig

    IHC staining of human liver using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human appendicitis using 66067-1-Ig

    IHC staining of human appendicitis using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human spleen using 66067-1-Ig

    IHC staining of human spleen using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human stomach cancer using 66067-1-Ig

    IHC staining of human stomach cancer using 66067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IF Staining of mouse kidney using 66067-1-Ig

    IF Staining of mouse kidney using 66067-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 66067-1-Ig

    IF Staining of human liver cancer using 66067-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of A549 using 66067-1-Ig

    IF Staining of A549 using 66067-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Featured Product
  • KD/KO Validated

Caveolin-1 Monoclonal antibody

Caveolin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66067-1-Ig

产品说明书

CloneNo. 6C2B2
引用文献(34)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig and More (4)

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (2)

别名

CAV1, Caveolin 1, 6C2B2, BSCL3, CAV

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, A431 cells, PC-3 cells, human heart tissue, pig heart tissue, Rat heart tissue, mouse heart tissue
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human liver tissue, human ovary cancer tissue, human placenta tissue, human tonsillitis tissue, human lung tissue, human appendicitis tissue, human stomach cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, mouse kidney tissue, human skin cancer tissue
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy Caveolin-1 Ready-To-Use IHC Kit. Caveolin-1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66067-1-Ig targets Caveolin-1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat, pig, canine, monkey, chicken, sheep
免疫原

CatNo: Ag8049

Product name: Recombinant human Caveolin-1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 11-179 aa of BC006432

Sequence: GHLYTVPIREQGNIYKPNNKAMADELSEKQVYDAHTKEIDLVNRDPKHLNDDVVKIDFEDVIAEPEGTHSFDGIWKASFTTFTVTKYWFYRLLSALFGIPMALIWGIYFAILSFLHIWAVVPCIKSFLIEIQCISRVYSIYVHTVCDPLFEAVGKIFSNVRINLQKEI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 caveolin 1, caveolae protein, 22kDa
别名 CAV1, Caveolin 1, 6C2B2, BSCL3, CAV
计算分子量 22 kDa
观测分子量 20-25 kDa
GenBank蛋白编号BC006432
基因名称 CAV1
Gene ID (NCBI) 857
RRIDAB_11043343
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ03135
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

实验方案

Product Specific Protocols
IF protocol for Caveolin-1 antibody 66067-1-IgDownload protocol
IHC protocol for Caveolin-1 antibody 66067-1-IgDownload protocol
WB protocol for Caveolin-1 antibody 66067-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Res

Inhibition of EGFR Overcomes Acquired Lenvatinib Resistance Driven by STAT3-ABCB1 Signaling in Hepatocellular Carcinoma

Authors - Beiyuan Hu
View Article
humanIF

Nat Commun

Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation

Authors - Jiayu Liu
View Article

Metabolism

Endothelial NPR-C promotes insulin resistance via Caveolin-1-mediated insulin transcytosis.

Authors - Zi-Qi Xu
View Article
mouseWB

Redox Biol

TrxR1 is involved in the activation of Caspase-11 by regulating the oxidative-reductive status of Trx-1

Authors - Dongsheng Bai
View Article
mouseIHC,IF

Adv Sci (Weinh)

Caveolin-1 Stabilizes SERCA2 to Counteract Acute Kidney Injury via Suppression of Ca(2+)-Dependent Endoplasmic Reticulum Stress in Distal Tubules.

Authors - Yan Zhang
  • KO Validated
View Article
humanIHC

Cell Death Dis

The methionine salvage pathway-involving ADI1 inhibits hepatoma growth by epigenetically altering genes expression via elevating S-adenosylmethionine.

Authors - Yu-De Chu
View Article
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